Mannosyl Alpha-1,3-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase (MGAT1)

There are over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The exon includes most of the 5-prime untranslated region, the complete coding sequence, and the complete 3-prime untranslated region. Southern blot analysis indicated that the gene exists in single copy in the human genome, and study of human-hamster somatic cell hybrids indicated that the gene is located on chromosome 5. GlcNAc-T I is expressed as 2 mRNAs of 3.1 and 2.7 to 3.0 kb in all tissues tested, although only the 3.1-kb mRNA is seen in brain, and expression levels are low.