Mastermind Like Protein 2 (MAML2)

The deduced 1,153-amino acid MAML2 protein has a calculated molecular mass of 125 kD. All 3 MAML proteins contain a conserved N-terminal basic domain, but the rest of the sequences are rather divergent. In addition, all 3 are proline- and glutamine-rich, and MAML2 and MAML3 contain several glutamine stretches. Northern blot analysis detected wide expression of MAML2 and MAML3 transcripts of about 7.5 kb. Fluorescence-tagged MAML proteins were expressed in a speckled nuclear staining pattern in transfected COS-7 and osteosarcoma cells, with considerable heterogeneity in the size of the dots.The deduced protein contains 1,173 amino acids. RT-PCR ELISA detected intermediate and relatively uniform MAML2 expression in all adult and fetal tissues and specific adult brain regions examined.