Mastermind Like Protein 3 (MAML3)

MAML3 contains 1,162 amino acids. RT-PCR ELISA detected high expression in ovary, with moderate to high expression in all other adult and fetal tissues and specific adult brain regions examined.The deduced 1,133-amino acid MAML3 protein has a calculated molecular mass of 115 kD. All 3 MAML proteins contain a conserved N-terminal basic domain, but the rest of the sequences are rather divergent. In addition, all 3 are proline- and glutamine-rich, and MAML2 and MAML3 contain several glutamine stretches. Northern blot analysis detected wide expression of MAML2 and MAML3 transcripts of about 7.5 kb. Fluorescence-tagged MAML proteins were expressed in a speckled nuclear staining pattern in transfected COS-7 and osteosarcoma cells, with considerable heterogeneity in the size of the dots.