Mediator Complex Subunit 11 (MED11)

MED11 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II.Using HeLa cells overexpressing epitope-tagged MED10, Sato et al. (2003) purified several components of the human Mediator complex, including MED11, which they called HSPC296, and they cloned the corresponding cDNA. Using in vitro-translated epitope-tagged proteins for protein-binding assays, Sato et al. (2003) found that MED11 directly interacted with the mediator subunit SURF5 (MED22). The MED11-SURF5 heterodimer could also be coimmunoprecipitated from cotransfected insect cells or Escherichia coli. Hartz (2008) mapped the MED11 gene to chromosome 16p13.2 based on an alignment of the MED11 sequence with the genomic sequence (build 36.1).