Mediator Complex Subunit 29 (MED29)

MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II , and general initiation factors.

The deduced 221-amino acid protein has a calculated molecular mass of 23.5 kD. It contains a PxxP motif for binding SRC homology-3 (SH3) domains. IXL is highly conserved across a wide range of species, and it shares 92% amino acid identity with mouse Ixl and 36% identity with Drosophila Intersex. Northern blot analysis detected a 3.6-kb transcript expressed at variable levels in all adult and fetal human tissues examined. Fluorescence-tagged IXL localized predominantly to the nucleus, but also to the cytoplasm, of transfected COS-7 cells.