Multiple EGF Like Domains Protein 11 (MEGF11)

Fluorescence-tagged MEGF11 was expressed in an irregular, mosaic-like pattern in transfected HEK293 cells, similar to that observed with MEGF10. However, unlike MEGF10, MEGF11 localized to lamellipodia in addition to the basal membrane of transfected cells.The transcript contains a repetitive element in its 3-prime end, and the deduced protein contains over 970 amino acids. MEGF11 has 17 EGF-like repeats and a transmembrane domain, and it shares significant similarity with rat Megf6 and human MEGF10. RT-PCR ELISA detected high expression in adult and fetal brain and adult spinal cord, moderate expression in adult kidney and ovary, low expression in heart, and little to no expression in other tissues examined. MEGF11 was expressed in all adult brain regions examined, with the highest level in cerebellum.