NADH Dehydrogenase Ubiquinone 1 Beta Subcomplex 8 (NDUFb8)

By a combination of EST database searching and PCR, Loeffen et al. (1998) isolated human cDNAs encoding NDUFB8 and 7 other complex I subunits. The predicted 186-amino acid human protein is 85% identical to ASHI, the bovine homolog.

Dunbar et al. (1997) mapped the NDUFB8 gene to 12q21 by fluorescence in situ hybridization (FISH). However, by intron-based radiation hybrid mapping, Emahazion and Brookes (1998) assigned the NDUFB8 gene to 10q23.2-q23.33. They stated that subsequent mapping studies with subfragments of the FISH-mapped recombinants of Dunbar et al. (1997) suggested chimerism and confirmed that the earlier FISH data were flawed.