NADPH Dependent Diflavin Oxidoreductase 1 (NDOR1)

NDOR1 encodes an NADPH-dependent diflavin reductase that contains both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) binding domains. NDOR1 catalyzes the transfers electrons from NADPH through FAD and FMN cofactors to potential redox partners. Alternative splicing results in multiple transcript variants. NR1 is a member of the FNR family of flavoenzymes that may play a role in the metabolic activation of bioreductive anticancer drugs and other chemicals activated by 1-electron reduction. NDOR1 overexpression enhanced cytotoxicity of the bioreductive anticancer agent, menadione, and that coexpression of DCPS reduced this toxicity. Western blot analysis showed increased DCPS protein levels in response to menadione, and immunoprecipitation assays showed that DCPS and NDOR1 interacted.