NADPH Oxidase Activator 1 (NOXA1)

NADPH oxidases (NOXs) catalyze the transfer of electrons from NADPH to molecular oxygen to generate reactive oxygen species (ROS). The NOX activator NOXA1 can stimulate both NOX1 and NOX2 (CYBB), but it appears to be more effective in activating NOX1.

The deduced 476-amino acid protein has a calculated molecular mass of 51 kD. It has 4 N-terminal tetratricopeptide repeat motifs, followed by a central activation domain, a PB1 (Phox and Bem1) domain, and a C-terminal SH3 domain. PCR analysis detected abundant p51-NOX expression in pancreas, with weaker expression in liver, kidney, spleen, prostate, small intestine, and colon. p51-NOX expressed in COS-7 cells had an apparent molecular mass of 53 to 54 kD by SDS-PAGE.