Neural Precursor Cell Expressed, Developmentally Down Regulated 4 (NEDD4)

Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.Nedd4 protein is localized to the cellular cytoplasm. the human NEDD4 protein has 86% amino acid identity with the mouse protein. It has homology to ubiquitin-protein ligases and contains 4 protein-protein interaction (WW) domains and a calcium/phospholipid binding domain.The deduced protein has a C2 domain and is homologous with mouse Nedd4, with which it shares 84% identity. Northern blot analysis detected NEDD4 in all tissues and cell lines examined, except brain.NEDD4 could also positively regulate PTEN through monoubiquitination of PTEN in human and mouse cells. Monoubiquitinated PTEN was stabilized by its accumulation in cell nuclei, and it retained its ability to antagonize AKT (AKT1) and cause apoptosis.