Nuclear Protein Localization Protein 4 (NPLOC4)

The deduced 660-amino acid human protein was predicted to be 34% identical to the yeast protein. RT-PCR analysis detected ubiquitous expression that was highest in brain, followed by spleen. Within brain, expression was strongest in amygdala and substantia nigra, followed by caudate nucleus.Sequence analysis predicted that the 608-amino acid transmembrane protein, which is 96% homologous to the rat protein, contains 5 potential N-glycosylation sites, 8 potential N-myristoylation sites, several phosphorylation sites, and a C-terminal zinc finger motif. Northern blot analysis detected a major transcript of 4.5 kb and minor transcripts of 9.5 and 2.5 kb. Expression was most abundant in brain, heart, skeletal muscle, kidney, and fetal liver. Yeast 2-hybrid analysis confirmed the interaction of UFD1L and NPL4 in human.