Nuclear Undecaprenyl Pyrophosphate Synthase 1 (NUS1)

Miao et al. (2006) found that the N-terminal domain of NOGOB induced chemotaxis in human umbilical vein endothelial cells (HUVECs). By screening a heart cDNAs expression library for genes that supported chemotaxis following transfection in COS or CHO cells, followed by database analysis, Miao et al. (2006) identified NGBR. The deduced 293-amino acid protein has an N-terminal signal peptide, a putative ectodomain, a type-1A transmembrane domain, and a long C-terminal cytoplasmic domain. Western blot analysis detected high Ngbr expression in mouse heart, liver, kidney, and pancreas.NOGOB and NGBR colocalized during angiogenesis induced by VEGF and wound healing in vivo. NOGOB and NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures.