Nuclear Valosin Containing Like Protein (NVL)

NVL.1 and NVL.2 encode 750- and 856-amino acid proteins, respectively, and the sequences of both are identical over the 750 C-terminal amino acids. Both proteins are hydrophilic without obvious membrane-spanning domains and contain 2 ATP-binding modules that are 51% identical.

NVL shows greatest similarity to the VCP subfamily of AAA proteins, including 35% amino acid identity with porcine VCP. However, the authors noted that NVLp is not the human ortholog of porcine VCP. NVL.2, but not NVL.1, contains a SRC kinase phosphorylation consensus sequence. In vitro expression of NVL.2 produced 95- and 110-kD proteins; the 110-kD form is likely phosphorylated. NVL.1 is approximately 83 kD and is not phosphorylated.