ORM1 Like Protein 1 (ORMDL1)

The deduced 153-amino acid protein has a calculated molecular mass of 17.4 kD. ORMDL1 contains 4 putative transmembrane domains and shares 83%, 84%, and 99% amino acid identity with ORMDL2 , ORMDL3, and mouse Ormdl1, respectively. It also share significant homology with its yeast orthologs, Orm1 and Orm2. RT-PCR showed ORMDL1 expression in all adult and fetal tissues examined. Northern blot analysis detected transcripts of 1.4, 2.5, and 3.3 kb in all tissues tested, with moderately high levels in pancreas, placenta, and brain and lowest levels in skeletal muscle and lung. Fluorescence-tagged ORMDL1 localized with an endoplasmic reticulum marker following transfection into COS-7 cells.ORMDL1 gene contains 5 exons and spans at least 17 kb. The first exon is untranslated.