PDZ And LIM Domain Protein 2 (PDLIM2)

PDLIM2 clones in retina, lens, retinal pigment epithelium/choroid, and fetal eye cDNA libraries. Northern blot analysis detected high expression in rat cornea and lower expression in sclera, lung, and combined trabecular meshwork, iris, and ciliary body. No expression was detected in retina. In situ hybridization of rat eye tissues detected Pdlim2 in corneal epithelial cells, but not in corneal stroma or endothelium or in other ocular tissues.

Pdlim2 coimmunoprecipitated with Actn1, Actn4, filamin A (FLNA), and myosin heavy polypeptide-9 (MYH9) in rat corneal and lung extracts. Myosin VI (MYO6) coimmunoprecipitated with Pdlim2 from corneal but not lung extracts. Alpha-actinins were the most abundant immunoprecipitated proteins.