PHD Finger Protein 19 (PHF19)

The PCL3L protein shares 41% and 44% sequence identity with human PCL1 (PHF1) and PCL2 (MTF2) proteins, respectively. The PCL family proteins contain a Tudor domain, 2 PHD fingers, a PCL homology domain, and a C-terminal tail. PCL3L also contains 2 putative nuclear localization signals. Northern blot analysis of multiple human tissues revealed expression of 2 major PCL3 transcripts of 1.0 and 4.8 kb at low levels in all tissues examined, with highest expression in thymus and heart. PCL3L was predominant in placenta, skeletal muscle, and kidney, and PCL3S was predominant in liver and peripheral blood leukocytes. Immunofluorescence microscopy revealed that PCL3S and PCL3L have distinct but overlapping localization patterns in the nucleus. A small amount of PCL3S was seen in the perinuclear cytoplasm.