PX Domain Containing Serine/Threonine Kinase (PXK)

The deduced 578-amino acid human PXK and 581-amino acid mouse Pxk share over 90% amino acid identity. PXK contains an N-terminal PX-like domain predicted to bind phosphoinositides, a putative protein kinase domain in the central region, which has greatest similarity to Drosophila slob, and a number of polyproline motifs. The authors identified a truncated PXK isoform in both human and mouse. RT-PCR analysis detected expression of the full-length Pxk in all mouse tissues examined and expression of the Pxk short isoform in all mouse tissues except skeletal muscle. Immunohistochemical studies detected Pxk expression throughout the nervous system in both mouse neurons and glia, including oligodendrocytes, astrocytes, microglia, and Schwann cells.