Pancreatic Lipase Related Protein 2 (PNLIPRP2)

PNLIPRP2 has 65% amino acid sequence identity with PNLIP, 63% identity with PNLIPRP1, and 20 to 30% identity with human gastric lipase, lipoprotein lipase, and hepatic lipase. Western blot analysis of recombinant PNLIPRP2 expressed in mammalian cells detected a secreted, approximately 50-kD protein; the authors concluded that PNLIPRP2 was glycosylated. In a standard pancreatic lipase assay, recombinant PNLIPRP2 showed lipolytic activity that was marginally increased by the addition of colipase and was inhibited by the lipase inhibitor orlistat. Northern blot analysis of pancreas RNA detected major 2.3- and 1.8-kb PNLIPRP2 transcripts. The expression level of PNLIPRP2 in pancreas was approximately 24-fold lower and 6-fold lower than the expression levels of PNLIP and PNLIPRP1, respectively.