Partitioning Defective 6 Homolog Gamma (PARD6g)

The deduced 376-amino acid protein contains a central CRIB motif and a PDZ domain. Northern blot analysis detected a 4.0-kb transcript expressed at highest levels in adult and fetal kidney and at lower levels in all other adult and fetal tissues examined.

PAR6-alpha, PAR6-beta, or PAR6-gamma could interact directly with GTP-bound RAC or CDC42 via their CRIB-like motif, and simultaneously with the atypical protein kinase C (aPKC) isoforms PKC-lambda/iota and PKC-zeta in an N-terminal head-to-head association. The ternary complexes were formed both in vitro and in vivo. When any of the PAR6 cDNAs and aPKC were expressed with a constitutively active form of RAC in HeLa or COS-7 cells, the proteins colocalized to membrane ruffles.