Phosphatidylinositol-4-Phosphate-5-Kinase Type I Gamma (PIP5K1g)

PIP5K1g encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.Pip5k1c has 2 alternative splice forms, consisting of 635 and 661 amino acids, that migrated at 87 and 90 kD by SDS-PAGE, respectively. The isoforms differ by the inclusion or exclusion of a 26-amino acid C-terminal tail. Northern blot analysis detected high levels of Pip5k1c expression in mouse brain, lung, and kidney.

AP2 via its mu-2 subunit (AP2M1) directly interacted with the kinase core domain of the PIPK gamma-subunit (PIP5K1C) in vitro and in native protein extracts. Endocytic cargo protein binding to mu-2 stimulated PIPK activity.