Phosphodiesterase 8A (PDE8A)

PDE8A gene contains 23 exons and spans over 80 kb. The 5-prime region of the PDE8A is GC-rich, and it contains a TATA box and numerous transcription factor binding sites, including some involved in cAMP-responsive transcriptional regulation and in expression of genes in T cells.

PDE8A is expressed as an approximately 4.5-kb mRNA in a wide variety of tissues. A recombinant protein containing the 545 C-terminal amino acids of PDE8A displayed very high affinity cAMP-specific PDE activity. Unlike PDE4 and PDE7, the other high affinity cAMP-specific PDEs, PDE8A was not inhibited by IBMX (3-isobutyl-1-methyl-xanthine).