Poly ADP Ribose Polymerase 9 (PARP9)

PARP9-transfected cell lines displayed 4-fold higher cell migration compared to controls and suggested that PARP9 may promote the migration and dissemination of high-risk diffuse B-cell lymphomas. Semiquantitative RT-PCR of 28 primary diffuse large B-cell lymphomas detected significantly higher PARP9 expression in high-risk lymphomas compared to low-risk lymphomas. Northern blot analysis of human tissues detected strong expression in skeletal muscle, spleen, colon, peripheral blood lymphocytes, lymph node, and fetal liver, with lower expression in heart, placenta, lung, adult liver, kidney, pancreas, thymus, prostate, testis, ovary, and small intestine. Immunofluorescence and subcellular fractionation studies localized PARP9 to the nucleus in both transfected NIH3T3 cells and B lymphocytes.