Poly ADP Ribose Polymerase 10 (PARP10)

Yu et al. (2005) identified PARP10 as a MYC-interacting protein by immunopurifying MYC-containing complexes from human Jurkat T cells. By searching an EST database using PARP10 peptide sequences, they identified a PARP10 cDNA. The deduced 1,025-amino acid protein had an apparent molecular mass of 150 kD by SDS-PAGE.

PARP10 contains an N-terminal RNA recognition motif, followed by a glycine-rich domain, a glutamic acid-rich domain, and a PARP catalytic domain. The glutamic acid-rich domain has a potential leucine-rich nuclear export signal (NES) and 2 ubiquitin-interacting motifs. Northern blot analysis detected a 3.8-kb PARP10 transcript in all tissues analyzed, with highest expression in spleen and thymus.