Potassium Channel Tetramerisation Domain Containing Protein 10 (KCTD10)

Zhou et al. (2005) cloned rat Kctd10, and by database analysis, they identified human KCTD10. The deduced 315-amino acid rat protein shares 97.7% identity with human KCTD10. The protein contains an N-terminal potassium channel tetramerization domain and a PCNA-binding motif near the C terminus. Northern blot analysis of rat tissues detected Kctd10 predominantly in lung with lower expression in heart and testis. Real-time PCR gave similar results and also detected lower Kctd10 expression in rat brain, liver, spleen, kidney, and skeletal muscle.rat Kctd10 interacted with the p50 subunit of DNA polymerase delta (POLD2) and with Pcna, suggesting that it functions in DNA replication or maintenance. The expression of Kctd10 increased in NIH3T3 cells following stimulation with TNF-alpha.