Processing Of Precursors 5 (POP5)

POP5. The deduced 163-amino acid protein has a calculated molecular mass of 18.8 kD. Western blot analysis of HeLa cells detected endogenous POP5 at an apparent molecular mass of 19 kD.

HeLa cell POP5 associated with RNase MRP and RNase P RNAs. The immunoprecipitated RNase P complex was able to cleave pre-tRNA into mature tRNA and its 5-prime leader sequence, indicating that POP5 is part of the catalytically active RNase P complex. Mutation analysis showed that the C terminus of POP5 was not required for association with RNase MRP or RNase P complexes or for RNase P activity.