Protein Kinase C And Casein Kinase Substrate In Neurons 2 (PACSIN2)

The complete human PACSIN2 encodes a deduced 486-amino acid protein that shares 93.6% sequence identity with mouse Pacsin2. The PACSIN2 proteins contain a CDC15 N-terminal domain, a C-terminal SH3 domain, 3 conserved regions specific to the PACSIN family, and 3 asn-pro-phe (NPF) motifs, which potentially bind to EH domains. The PACSIN2 proteins share high sequence similarity with chicken FAP52 and mouse Pacsin1. Pacsin2 expression in all tissues examined, with the highest levels in brain, heart, skeletal muscle, and ovary. Immunofluorescence microscopy of recombinant Pacsin2 expressed in fibroblasts showed a broad, vesicle-like cytoplasmic distribution that appeared to partially overlap with the distributions of both the actin filament and microtubule networks.