Protein O-Linked Mannose Beta-1,2-N-Acetylglucosaminyltransferase (POMGNT1)

The POMGNT1 gene encodes a 660-amino acid type II transmembrane protein, with the region from phe38 to ile58 constituting a putative transmembrane domain. Northern blot analysis detected a 2.7-kb transcript in all 23 normal tissues tested, indicating that human POMGNT1 is constitutively expressed. An additional weaker 3.4-kb band was observed in spinal cord, lymph node, and trachea, suggesting the presence of 2 transcriptional initiation sites or alternative splicing in these tissues.The human and mouse proteins share 99% identity over 620 residues. Northern blot analysis revealed wide expression of a 3.3-kb transcript.GNTI.2 is specific for alpha-linked terminal Man and does not have MGAT3, MGAT4, MGAT5, MGAT7, or MGAT8 activity.