Proteoglycan 3 (PRG3)

Through large-scale sequencing, Plager et al. (1999) identified PRG3 as one of several transcripts upregulated in umbilical cord precursor cells following stimulation with interleukin-5. Using this sequence as probe, Plager et al. (2001) cloned PRG3 from a phage genomic library of placental tissues. Plager et al. (1999) found that the cDNA sequence predicts a mature 117-amino acid protein with a prepro- region of 108 amino acids. PRG3 shares 49% amino acid sequence identity with PRG2. RT-PCR demonstrated that PRG3 is expressed exclusively in bone marrow. Protein purified from eosinophil granules showed a molecular mass of 13.4 kD.Plager et al. (1999) found that the biologic activity of PRG3 is similar to that of PRG2 in cell killing and in neutrophil and basophil stimulation assays, but usually with reduced potency.