Prune Homolog 2 (PRUNE2)

The deduced 2,714-amino acid protein has a coiled-coil sequence, a proline-rich region, a P loop, and a C-terminal BNIP2 and CDC42GAP (ARHGAP1) homology (BCH) domain, which contains 3 putative transmembrane domains. Northern blot analysis detected a 12-kb transcript in fetal brain. Semiquantitative RT-PCR revealed BMCC1 expression in all tissues examined except bone marrow, thymus, and spleen.

Expression was highest in brain, cerebellum, spinal cord, and adrenal gland. RT-PCR of synchronized HeLa cells showed that BMCC1 was predominantly expressed in G1 phase of the cell cycle. In situ hybridization of mouse embryos showed that Bmcc1 was specifically expressed in neural tube and neural crest-related tissues.