Pyridoxal Phosphatase (PDXP)

Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP

The deduced 296-amino acid protein has a calculated molecular mass of 31.7 kD. PDXP contains 2 N-glycosylation sites, 7 phosphorylation sites, and 4 N-myristoylation sites. Gel filtration analysis showed that recombinant PDXP expressed in E. coli formed dimers of about 60 kD. Northern blot analysis detected a 2.1-kb transcript expressed at variable levels in all adult tissues examined. RNA dot blot analysis detected ubiquitous expression, with highest levels in fetal brain and all adult brain regions examined. High expression was also detected in liver and testis.