RAN Binding Protein 10 (RANBP10)

RanBP10, as a factor that binds beta-tubulin and associates with megakaryocyte microtubules. Unexpectedly, RanBP10 harbors GEF activity toward Ran.

A point mutation in the candidate GEF domain abolishes exchange activity, RanBP10 as a localized cytoplasmic Ran-GEF. RNA interference-mediated loss of RanBP10 in cultured megakaryocytes disrupts microtubule organization. Spatiotemporally restricted generation of cytoplasmic Ran.GTP may influence organization of the specialized microtubules required in thrombopoiesis and that RanBP10 might serve as a molecular link between Ran and noncentrosomal microtubules. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules.