RAN Guanine Nucleotide Release Factor (RANGRF)

The deduced protein contains 185 amino acids. Epitope-tagged Mog1 localized to nuclei of transfected baby hamster kidney cells, but it was excluded from nucleoli.

MOG1a and MOG1b share 29% and 30% amino acid identity with S. cerevisiae Mog1, respectively. Northern blot analysis detected a 0.85-kb MOG1a transcript and a 1.1-kb MOG1b transcript in all tissues examined, with MOG1a being the predominant transcript. PCR of a breast cancer cell line showed multiple products, suggesting the presence of additional splice variants. SDS-PAGE of in vitro translated MOG1a indicated that the protein migrates with an apparent molecular mass of about 28 kD. Transfection of fluorescence-labeled MOG1 into HEK293 cells resulted in overall staining with a nuclear concentration.