RNA Binding Motif Protein 6 (RBM6)

The deduced 1,123-amino acid major protein contains 20 inexact 6-amino acid repeats close to the N terminus; 2 RNA-binding domains; 2 zinc finger motifs; a bipartite nuclear localization signal; and a 4-residue nuclear localization pattern. They noted that all of the predicted functional domains in RBM6 are also present in RBM5 , with which it shares 30% identity. Northern blot analysis detected several RBM6 transcripts, ranging from approximately 3 to 4.4 kb, in all tissues examined. Highest expression was in liver, with intermediate expression in placenta, lung, skeletal muscle, and kidney. Southern blot analysis showed no evidence of homozygous deletion of RBM6 in tumor cell lines, the gene being partially deleted in only 1 of 9 lung cancer cell lines.