RNA Polymerase II Associated Protein 2 (RPAP2)

Using affinity purification, mass spectrometry, and bioinformatic analysis, Jeronimo et al. (2007) mapped an interaction network of protein complexes involved in the RNA polymerase II machinery. They found that RPAP1, RPAP2, RPAP3, and XAB1 were tightly bound to RNA polymerase II in the soluble nuclear compartment in HEK293 cells. Examination of the protein interaction network revealed that the RPAPs and XAB1 also interacted with components of the Mediator and Integrator complexes, as well as with proteins able to assemble into molecular chaperone complexes, including the prefoldins and the AAA+ ATPases RUVBL1 and RUVBL2).The deduced protein contains 612 amino acids.