RNA Polymerase II, DNA Directed Polypeptide F (POLR2F)

POLR2F encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template.The deduced 127-amino acid POLR2F protein has a calculated molecular mass of approximately 14.4 kD. It is highly acidic, with a calculated isoelectric point of 3.7. POLR2F contains a predicted C-terminal leucine zipper domain, which is not preceded by a basic domain. The POLR2F protein shares 49% sequence identity with the S. cerevisiae polymerase subunit ABC23, with the C-terminal regions showing the highest conservation.