RUN And FYVE Domain Containing Protein 3 (RUFY3)

The deduced proteins in all 3 species have 469 amino acids and contain a RUN domain. The mouse and rat proteins contain a splice variant, designated singar2, of 487 amino acids. Expression of singar1 in rat brain was relatively low on embryonic day 15, peaked around postnatal day 4, and decreased in the adult brain. Immunocytochemical analysis showed that rat singar1 and singar2 accumulate in growth cones of minor processes and axons.

Reduction of singar1 expression by RNAi led to an increase in the population of neurons bearing surplus axons. Overexpression of singar1 suppressed the formation of surplus axons induced by excess amounts of shootin1 , without affecting normal polarization processes.