RAB Interacting Lysosomal Protein (RILP)

RILP is expressed at varying levels in all tissues examined. Expression was highest in adult heart, stomach, adrenal gland, thyroid gland, salivary gland, liver, and lung, and in fetal heart and liver. Expression was lowest in whole fetal brain and in all adult brain regions. Northern blot analysis confirmed ubiquitous expression of 0.9- and 1.8-kb transcripts. Expression levels of the 2 transcripts varied between tissues.The deduced 401-amino acid protein has a calculated molecular mass of about 45 kD and contains 2 coiled-coil regions. RILP shares significant homology with a mouse protein belonging to the ezrin-radixin-moesin (ERM) family. Northern blot analysis detected transcripts of about 1.2 and 1.8 kb in the 3 tissues tested (lung, spleen, and stomach). Western blot analysis detected a 50-kD RILP protein in all cell lines examined.