Rabphilin 3A Like Protein (RPH3AL)

Noc2 interacts with the LIM domain-containing protein zyxin, a component of the cytoskeleton. A partial rabphilin-3A-related protein was identified and designated Noc2; a full-length Noc2 clone was isolated from a rat insulin-secreting cell line cDNA library. The Noc2 cDNA encodes a 302-amino acid protein that shows 40.7% amino acid identity and 77.9% similarity to the N-terminal region of rat rabphilin-3A. However, it lacks the C2 domains, and hence the Noc2 designation. Noc2 contains 1 potential protein kinase A and 3 potential protein kinase C phosphorylation sites. RNA blot analysis revealed that a single 2.2- or 2.6-kb transcript of Noc2 is expressed at very high levels in pancreatic islets, and at low to moderate or high levels in other endocrine tissues and hormone-secreting cell lines, but at very low levels in brain.