Ras Related GTP Binding Protein A (RRAGA)

RRAGA shares similarity with RAS (HRAS) and contains phosphate/magnesium-binding motifs PM1, PM2, and PM3, and the guanine nucleotide-binding motif G1. An additional guanine nucleotide-binding motif, G2, is strikingly different from those found in all other RAS homologs. The C-terminal domain contains a putative transmembrane region. Northern blot analysis of rat tissues detected highest expression in adrenal gland.Mutation analysis revealed N- and C-terminal domains of FIP1 that were required for the interaction. In human embryonic kidney cells, TNF-alpha (TNF), a proinflammatory cytokine with antiviral activity, promoted the transient association of FIP1 with phosphoproteins. In mouse fibroblasts, antisense FIP1 RNA inhibited TNF-alpha-induced cytolysis.