RecQ Mediated Genome Instability Protein 1 (RMI1)

RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions.deduced 625-amino acid protein contains an N-terminal nucleic acid binding domain and a C-terminal domain conserved across several eukaryotic species.BLAP75 was associated with all 3 BLM-containing protein complexes immunopurified from HeLa cells. BLAP75 colocalized with BLM in subnuclear foci in response to DNA damage, and its depletion by small interfering RNA (siRNA) impaired the recruitment of BLM to these foci. Depletion of BLAP75 also resulted in deficient BLM phosphorylation during mitosis as well as defective cell proliferation. Cells depleted of BLAP75 displayed an increased level of sister-chromatid exchange, similar to cells depleted of BLM.