Regulator Of Chromosome Condensation 1 (RCC1)

RCC1 was found to have 14 exons, 8 of which (starting from the seventh one) coded the 7 repeated sequences of RCC1 protein. A single exon corresponded roughly to each repeat of the RCC1 protein except for the middle one, indicating that the RCC1 gene was generated through amplification of a primordial exon. Primer extension analysis demonstrated the presence of an internal promoter.the nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for RAN, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. In the RCC1-depleted egg extracts, RanGTP, but not RanGDP, induced self-organization of microtubule asters, and the process required the activity of dynein.