Reticulon 4 Receptor Like Protein 1 (RTN4RL1)

NGRH2 shares 48% similarity with NGR and 52% similarity with NGRH1 (RTN4RL2). Probing a brain multitissue array revealed NGRH2 highly expressed in all cerebral cortex regions, in amygdala, hippocampus, accumbens nucleus, thalamus, and pituitary. Expression was lower in other tissues examined.Weaker or no expression was detected in other brain regions. In situ hybridization of rat brain detected Ngrh2 in several cortical layers and in large cell bodies typical for pyramidal neurons. Immunohistochemical analysis found Ngrh2 in neuronal cell bodies and axons. Digestion with phospholipase C confirmed that NGRH2 has a GPI anchor, and sucrose density fractionation of transfected Chinese hamster ovary (CHO) cells demonstrated the association of human NGRH2 with lipid rafts.