Retinoic Acid Induced Protein 14 (RAI14)

Human RAI14 has a predicted molecular mass of 110 kD, a pI of 5.83, 6 potential N-glycosylation sites, several ankyrin repeats, and several coiled-coil helical domains. Like the ARPE-19 cells, placenta showed an intense signal at 5 kb and a weak signal at 3 kb; the reverse was observed in testis, with an intense signal at 3 kb and a weak signal at 5 kb. RAI14 was also highly expressed in several human cancer cell lines. Confocal microscopic analysis in ARPE-19 cells showed threadlike projections in the cytoplasm reminiscent of the cytoskeleton. The expression of Rai14 was detected in mouse embryo at embryonic day 9.5 by in situ hybridization, and the expression appeared to be developmentally regulated. In adult mouse, the highest level of expression was detected in the seminiferous tubules of testis.