Rififylin (RFFL)

The mouse Rffl cDNA encodes 2 proteins of 336 and 364 amino acids, generated by alternative splicing. The N-terminal region contains a domain related to the FYVE double zinc finger domain that mediates binding to phosphatidylinositol 3-phosphate (PIK3), and the C-terminal region contains a consensus sequence for a C3HC4 RING finger domain.

Northern blot analysis revealed a ubiquitously expressed transcript of approximately 4 kb and a smaller transcript of about 2 kb at a very high level in testis and at a moderate level in liver. RT-PCR indicated that both proteins are ubiquitously expressed, with the larger isoform almost exclusively expressed in adult mouse liver and kidney cells and in HeLa cells.