Ring Finger Protein 216 (RNF216)

TRIAD3, contain 2 RING fingers flanking a conserved cysteine-rich (C6HC) zinc-binding domain called a double RING finger-linked (DRIL) domain. Like other RING finger proteins, TRIADs interact with a variety of proteins. The predicted 488-amino acid protein contains 4 central RING-like domains (RLDs) and a C-terminal proline-rich domain. Northern blot analysis revealed ubiquitous expression of 3.0- and 6.0-kb transcripts, with highest levels in peripheral blood leukocytes and testis. Western blot analysis showed a 56-kD protein in several human cell lines. Immunofluorescence microscopy suggested a cytoplasmic localization for ZIN, like that of RIP.ZIN also potentiated RIP-induced apoptosis. Coimmunoprecipitation experiments showed that ZIN did not compete with TRAF2 for interaction with RIP.