Ring Finger Protein 24 (RNF24)

RNF24 binds TRPC6 and other transient receptor potential cation channel family members and is involved in regulation of the trafficking and insertion of TRPCs into the plasma membrane. Sodium carbonate treatment and immunofluorescence confocal microscopy, determined that RNF24 is an integral membrane protein localized primarily to the Golgi apparatus. Biotinylation and confocal laser-scanning microscopy studies showed that RNF24 plays a role in reduction of trafficking and insertion of TRPCs into the plasma membrane. Pulsed-chase experiments and TRPC6 activation assays showed that RNF24 was not involved in the maturation or degradation of TRPC6 and did not regulate TRPC activity.