RuvB Like Protein 1 (RUVBL1)

RUVBL1 coimmunoprecipitated with several cellular proteins and was present in the RNA polymerase II holoenzyme complex purified over multiple chromatographic steps.

The calculated molecular mass and pI of the predicted 456-amino acid protein were close to the experimentally determined values of 54 kD and 6.5. By immunofluorescence, Holzmann et al. (1998) determined that NMP238 is found primarily in the nucleus, although it is also present in the cytoplasm. The NMP238 staining pattern appeared as punctate signals in both in situ prepared nuclear matrices and in the nucleoplasm of whole cells. Northern blot and RNA dot blot analysis revealed that NMP238 is expressed ubiquitously as an approximately 1.8-kb mRNA.