SLIT ROBO Rho GTPase Activating Protein 1 (SRGAP1)

SLIT increased SRGAP1-ROBO1 interaction and inactivated CDC42. A dominant-negative SRGAP1 blocked SLIT inactivation of CDC42 and SLIT repulsion of migratory cells from the anterior subventricular zone of the forebrain. A constitutively active CDC42 blocked the repulsive effect of SLIT. These results demonstrated important roles for GAPs and CDC42 in neuronal migration.The 1,051-amino acid KIAA1304 protein is 48% identical to C1 RHOGAP and 68% identical to KIAA0456. It was predicted to be involved in cell signaling/communication. RT-PCR analysis detected moderate expression of KIAA1304 in all tissues tested except skeletal muscle, pancreas, and spleen, where expression was low. KIAA1304 was expressed in all brain regions tested.