SLIT ROBO Rho GTPase Activating Protein 3 (SRGAP3)

SRGAP3 encodes a 650-amino acid protein that is 48.1% identical to C1 RHOGAP. It was predicted to be involved in cell signaling/communication. RT-PCR analysis detected low-level expression of KIAA0411 in brain, lung, kidney, thymus, prostate, testis, and ovary, with little or no expression in all other tissues tested.Mutation analysis revealed that trp757 in the SH3 domain of WRP and proline-rich sequences within residues 320 to 332 and 420 to 433 of WAVE1 were required for the interaction. Recombinant WRP stimulated intrinsic GTPase activity of Rac, but had no effect on Rho or CDC42. WRP also reduced neurite outgrowth when transfected into rat cerebellar granular neurons, and this effect was not dependent upon interaction with WAVE1.