SNF8, ESCRT-II Complex Subunit Homolog (SNF8)

SNF8, VPS25, and VPS36 form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL) .

Eap30 and Eap20 (VPS25) could be coimmunoprecipitated in the absence of Eap45 (VPS36), and that Eap20 and Eap45 could be coimmunoprecipitated in the absence of Eap30. However, little Eap30 was coimmunoprecipitated with Eap45 in the absence of Eap20. recombinant EAP30 could interact with recombinant ELL in vitro and derepress the inhibitory activity of ELL against RNA polymerase II.